The Dietzel group has a history of research in the field of nuclear organization. While the human genome is sequenced now for several years, its cell biology is yet mostly unknown. We investigate the principles according to which chromosomes and gene loci are distributed in the nuclear space. That includes the comparison of the distribution of transgenes in undifferentiated and differentiated cells. We also look for endogeneous genes to answer the question "Are acitve and inactive genes differently distributed in the nculeus?". Here, we follow genes during differentiation, looking for genes being inactive in an undifferentiated cell type and active in a differentiated cell type or vice versa.

Techniques applied are fluorescence in situ hybridization (FISH), GFP-tagging of transgenes, three-dimensional wide field microscopy, confocal laser scanning microscopy and computerized quantitative image analysis.

Since Steffen started at the Walter-Brendel-Zentrum (WBex), the focus moves to high resolution microscopy of blood vessels and blood cell differentiation, in particular Multi Photon Microscopy (see respecting Wikipedia articles: English / German).

See also the list of finished theses from our group and our recent publications.

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