Dietzel, S., H. Niemann, B. Brückner, C. Maurange and R. Paro (1999).
The nuclear distribution of Polycomb during Drosophila melanogaster development
shown with a GFP fusion protein.
Chromosoma
, 108(2):83-94. (
Abstract)
If you are not coming from there anyway, you might want to check out the
introduction page
.
![]() |
Overview over a region in the adult testis with several spermatocytes. Single confocal sections of DNA, Polycomb and RNA and the Overlay of the three. The very bright DNA signals on the right edge of the image (pink in the overlay) are the nuclei of testis wall cells. |
![]() |
Click here
to enlarge this image. (906x688 pixel, 654 Kbytes) |
Consecutive confocal sections through two spermatocyte nuclei from the testis shown in the previous image. The distance between two sections is 2.1 µm. To facilitate the visualization of overlap between DNA and Polycomb, DNA was false colored in red (1st and 3rd row), Polycomb in green (2nd and 3rd row) and RNA in blue (4th row). Note the high overlap of DNA and Polycomb for the autosomes (arrowheads) and the limited overlap for the nucleolar X-chromosome. The identity of the nucleolus was confirmed by RNA-staining. |
![]() |
|
Confocal section of two mature spermatocytes with pronounced evaginations. Note the high overlap of DNA and Polycomb for the autosomes in the evaginations and the limited overlap for the nucleolar X-chromosome. In the lower nucleus the Polycomb domain of one autosome is nearly completely out of focus. In neighboring sections it is detectable in the upper left corner of that nucleus. | |
Quicktime movie showing a 3D rotation
of these two nuclei. (very nice! 669 Kbytes.) Left: DNA stain (DAPI). Right: Polycomb - GFP signal. If the movie is not displayed automatically below, you can reload
this page with 3 embedded Quicktime movies by clicking
here
. The combined size of the 3 is about 2.5 Mbytes. Or
click here
to download only this movie. |
Quicktime movie showing serial sections
of another nucleus (608 Kbytes.) Left: DNA stain (DAPI). Center: Polycomb - GFP signal. Right: RNA stain (Propidium Iodide). Note the dispersed DNA around the spherical Polycomb signal of the nucleolus (presumably the X-chromosome) and the more compact appearance of the 2 other DNA/Polycomb domains (presumably the bivalents of the autosomes). If the movie is not displayed automatically below, you can reload
this page with 3 embedded Quicktime movies by clicking
here
. The combined size of the 3 is about 2.5 Mbytes. Or
Click here
to download only this movie. |
Quicktime movie showing a 3D rotation
of this other nucleus. (1220 Kbytes) Left: DNA stain (DAPI). Center: Polycomb - GFP signal. Right: RNA stain (Propidium Iodide). See above for description If the movie is not displayed automatically below, you can reload
this page with 3 embedded Quicktime movies by clicking
here
. The combined size of the 3 is about 2.5 Mbytes. Or
Click here
to download only this movie. |
Confocal section of onion stage spermatids in an unfixed adult testis. Only
a weak fluorescent signal little above background remained (left). The onion
pattern of the Nebenkern can be identified in the corresponding brightfield
image (right).
Head of a mature spermatid bundle. Projection of fluorescence images (left)
and on corresponding DIC section. No Polycomb signal can be detected.