Dietzel, S., H. Niemann, B. Brückner, C. Maurange and R. Paro (1999).
The nuclear distribution of Polycomb during Drosophila melanogaster development shown with a GFP fusionprotein. Chromosoma , 108(2):83-94. ( Abstract and pdf-Article available at the journal's Web site. (pdf only if your institution subscribes to idealibrary.) Abstract available at " PubMed ") |
The material presented here is meant as an addendum to this article.
It contains Quicktime movies and images that didn't make it in the paper,
mainly due to space restrictions. A very very short introduction is given
below. For more detailed information about our findings and our interpretations
please read the article.
The material is grouped in three areas:
Visualization of the development of Drosophila embryos with a Polycomb-GFP fusionproteinIn the four movies presented here, PC-GFP is used as a nuclear marker to follow the development of the embryo over time, starting shortly before blastoderm stage. Two movies show the embryo from the blastula stage to the hatching of the larvae while the two others focus on the early stages. |
Distribution of Polycomb during blastoderm mitosisThis page contains only one Quicktime movie, but a very nice one. It shows the distribution of the Polycomb-GFP fusion protein during two nuclear cycles in the blastoderm stage in high temporal and spatial resolution. File size of the movie: ~2.4 MBytes. |
Distribution of Polycomb during spermatogenesisExamples for the distribution of Polycomb in larval testis (1 image) and adult testis are given.This page is available in two versions: Version 1 contains "only" 8 images in various sizes. Version 2 contains in addition 3 embedded Quicktime movies with a combined (additional) file size of ~2.5 MBytes. Version 1 gives you the possibility to switch to Version two if you decide later that you want the movies. |
MethodsFor detailed methods see the paper. The GFP used was EGFP from Clontech , a variety of the S65T mutation (see here for spectrum). Confocal images were recorded with the Leica TCS NT microscope of the ZMBH except otherwise noted. GFP was excited with 488 nm, a dichroic mirror at 500 nm and a bandpass 500 - 550 nm were used. The images were transferred from the microscope to a Mac, further processed in NIH Image and saved as Quicktime Movies or combined to RGB images in Adobe Photoshop. |