Distribution of Polycomb - GFP fusion proteins
in cell nuclei of Drosophila -
Movies and Images
During my time as a Post-Doc in the
Lab of Renato Paro
at the ZMBH
in Heidelberg, Germany, I had the opportunity to study fly lines transgenic
for a Polycomb-GFP fusion protein under the microscope. The lines were generated
by Hartmut Niemann or Bodo Brückner in the Paro Lab. The results are
described in the following article:
|Dietzel, S., H. Niemann, B. Brückner, C. Maurange and R. Paro (1999).
The nuclear distribution of Polycomb during Drosophila melanogaster
development shown with a GFP fusionprotein.
, 108(2):83-94. (
and pdf-Article available at the journal's Web site. (pdf only if your institution
subscribes to idealibrary.) Abstract available at "
The material presented here is meant as an addendum to this article.
It contains Quicktime movies and images that didn't make it in the paper,
mainly due to space restrictions. A very very short introduction is given
below. For more detailed information about our findings and our interpretations
please read the article.
Polycomb (PC) is a nuclear protein involved in the stable and heritable silencing
of a variety of developmentally important genes. For more information about
Polycomb see the Web site
of the Paro Lab
. GFP stands for green fluorescent protein. For more information about GFP
. For more information about Drosophila, check the
Drosophila virtual library.
The material is grouped in three areas:
In the four movies presented here, PC-GFP is used as a nuclear marker to
follow the development of the embryo over time, starting shortly before blastoderm
stage. Two movies show the embryo from the blastula stage to the hatching
of the larvae while the two others focus on the early stages.
This page contains only one Quicktime movie, but a very nice one. It shows
the distribution of the Polycomb-GFP fusion protein during two nuclear cycles
in the blastoderm stage in high temporal and spatial resolution. File size
of the movie: ~2.4 MBytes.
Distribution of Polycomb during spermatogenesis
Examples for the distribution of Polycomb in larval testis (1 image) and
adult testis are given.
This page is available in two versions: Version 1
contains "only" 8 images in various sizes.
contains in addition 3 embedded Quicktime movies with a combined
(additional) file size of ~2.5 MBytes. Version 1 gives you the possibility
to switch to Version two if you decide later that you want the movies.
For detailed methods see the paper. The GFP used was EGFP from
, a variety of the S65T mutation (see
for spectrum). Confocal images were recorded with the
TCS NT microscope of the ZMBH
except otherwise noted. GFP was excited with 488 nm, a dichroic mirror
at 500 nm and a bandpass 500 - 550 nm were used. The images were transferred
from the microscope to a Mac, further processed in
and saved as Quicktime Movies or combined to RGB images in
Technical note about the drosophila area on this web-site:
(You don't have to read this...)
I first set up this area while I was in Urbana on the local University server.
When I moved from Urbana to Munich , I had to reconfigure the whole site.
What's much worse: The URLs that some people had bookmarked and links from
other Web-sites became suddenly invalid. That was one of the reasons why
I got my own domain. However, the available space on this domain is small
(1 Mbyte) and I cannot possibly store movies or even images on it. I solve
this problem thus that the text pages are stored on dietzellab.de while the
actual data files are loaded from the web server of my institution (currently
the University of Munich, see homepage). This way a permanent Web-address
is available without a big price tag for me.
Last Update: 4. September 2000